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Fertility & Reproductive Health

Before Artificial Fertilisation Begins: The Tests Both Partners Complete and Why They Matter

25 min read
Before Artificial Fertilisation Begins: The Tests Both Partners Complete and Why They Matter

Key Takeaways

  • The NHS estimates about one in seven couples may have difficulty conceiving, while roughly 84 in 100 couples conceive within a year of regular unprotected sex.
  • IUI depends on at least one open fallopian tube, so a tubal patency test (HSG or HyCoSy) often decides whether insemination or IVF is the sensible route.
  • AMH and antral follicle count estimate how many eggs remain, not their quality, and are used mainly to choose a stimulation plan and reduce over-response risk.
  • The WHO laboratory manual recommends two to seven days without ejaculation before a semen analysis, and the NHS advises repeating an abnormal result before acting on it.
  • A mock embryo transfer measures uterine depth and the cervical path so the real transfer is smoother; it does not test fertility or predict implantation.
  • The NHS lists HIV, hepatitis B and hepatitis C screening as standard for both partners before IVF, and results often have an expiry date that may require repeat testing after a pause.
Quick Answer

Before IUI or IVF starts, both partners usually complete a structured workup: a semen analysis for the male partner; hormone blood tests, an ultrasound of the ovaries and uterus, and often a tubal patency check for the female partner; and infection screening (HIV, hepatitis B and C, sometimes syphilis, chlamydia and rubella immunity) for both. Some IVF programs add a mock embryo transfer. Results shape the treatment plan, which the care team finalizes with you.

The folder sits on the kitchen table for a week before anyone opens it. Inside is a checklist from the fertility clinic: two pages for her, one for him, a shared page of blood tests, and a line at the bottom that reads “return all results before scheduling.” It looks less like the start of something hopeful and more like the paperwork for a mortgage.

That reaction is common, and understandable. Couples arrive expecting to talk about treatment and instead spend the first weeks talking about tests. Yet the artificial fertilisation preparation tests are not an obstacle placed in front of the real work; they are the real work. Each one answers a question the treatment cannot answer on its own.

This explainer walks through what those tests are, who completes them, what the results change, and which parts of the folklore around them do not survive contact with the evidence.

What are artificial fertilisation preparation tests, and what does the term cover?

“Artificial fertilisation” is an umbrella phrase rather than a medical diagnosis. Clinicians tend to split it into two families. Artificial insemination places prepared sperm inside the reproductive tract so fertilization happens in the body; in vitro fertilization (IVF) combines eggs and sperm in a laboratory dish and returns an embryo to the uterus later. The NHS and the Mayo Clinic describe both as forms of fertility treatment, and both begin with the same question: what, if anything, is making conception difficult?

The artificial fertilisation preparation tests exist to answer that question with data rather than guesswork. They fall into three broad groups. First, tests that look at eggs, ovulation and the reproductive anatomy of the female partner. Second, tests that look at sperm and the reproductive health of the male partner. Third, tests both people complete, mainly infection screening and general blood work, because an embryo or a pregnancy is affected by the health of whoever carries it and whoever contributes gametes.

A word on scale. The NHS estimates that around one in seven couples may have difficulty conceiving, while roughly 84 in 100 couples conceive naturally within a year of regular unprotected sex. Testing before treatment sorts the remaining group into recognizable patterns: ovulation that is not happening reliably, blocked fallopian tubes, low sperm count or movement, a uterine cavity that needs attention, or the frustrating label of “unexplained,” where every result looks normal yet pregnancy has not happened.

Why does that matter? Because IUI and IVF are not interchangeable. IUI needs at least one open fallopian tube and a workable sperm sample. IVF does not need open tubes but does need retrievable eggs. Without the tests, a couple could sit through several cycles of a treatment that was never going to suit their situation.

What are the four types of artificial insemination, and how do they differ from IVF?

People searching this question usually find four names. Intrauterine insemination (IUI) places washed, concentrated sperm directly into the uterus through a thin catheter, a flexible tube passed through the cervix. Intracervical insemination (ICI) places sperm at the opening of the cervix, closer to what happens during intercourse. Intravaginal insemination deposits sperm in the vagina and is the method used in some at-home donor arrangements. Intratubal insemination places sperm directly into a fallopian tube; it is rarely performed today because it adds complexity without clear benefit over IUI.

Doctor consulting patient, holding specimen container: What are the four types of artificial insemination, and how do they d

In practice, when a clinic says “artificial insemination,” it almost always means IUI. The Mayo Clinic describes the procedure as short, usually a few minutes, performed around the time of ovulation, with or without medicines that encourage an egg to mature. The sperm “washing” step separates moving sperm from seminal fluid, which would cause painful cramping if placed directly into the uterus.

IVF is a different animal. Eggs are stimulated to mature, collected under sedation with a needle guided by ultrasound, combined with sperm in the laboratory, and one resulting embryo is later transferred into the uterus. Intracytoplasmic sperm injection (ICSI), where a single sperm is injected into each egg, is a laboratory variant often used when sperm quality is the main issue.

The preparation tests overlap heavily but not completely. Every route needs a semen analysis and infection screening. IUI specifically depends on confirming that at least one tube is open, since sperm still has to travel to meet the egg. IVF leans harder on ovarian reserve testing, because the plan depends on how many eggs the ovaries can be expected to release in response to stimulation. Knowing which route you are being assessed for helps make sense of why certain tests appear on your list and others do not.

How the workup actually happens, from first appointment to the green light

Most couples describe the process as three visits stretched over one to two menstrual cycles, although the exact rhythm depends on the clinic and on which tests need a specific day of the cycle.

The first appointment is mostly conversation. A clinician takes a history from both partners: how long you have been trying, cycle regularity, previous pregnancies or losses, surgeries, infections, medicines, and habits such as smoking. The NHS notes that this history alone often points toward the likeliest explanation before a single result comes back. A physical examination may follow, and the test requests are issued.

The second phase is the testing itself, and timing is where it gets fiddly. Some female hormone tests are drawn in the first few days of the period; a progesterone test to confirm ovulation is drawn about a week before the next period is due. A tubal patency check, which looks for blockages in the fallopian tubes, is usually scheduled after bleeding stops but before ovulation. The semen analysis can be done any day, but requires a short abstinence window beforehand. Infection screening bloods can be drawn at any point.

The third visit is the results discussion. This is where the artificial fertilisation preparation tests turn into a plan. A clinician explains each finding in context, describes which treatment route fits, and outlines what a cycle would involve. If something unexpected appears, such as a fibroid inside the uterine cavity or a very low sperm count, the plan may branch: a further test, a referral, or a recommendation to treat a separate problem first.

Consent and counseling sit alongside all of this. Fertility treatment carries emotional weight, and many programs offer or require a conversation with a counselor, particularly where donor sperm or eggs are involved. None of these steps is padding. Each closes off a question that would otherwise remain open during treatment.

Who is usually offered treatment promptly, and who is usually asked to wait

The answer turns on two things: what the tests show, and whether waiting would make the outlook worse.

Healthcare professional consulting with female patient indoors: Who is usually offered treatment promptly, and who is usuall

Couples usually move toward treatment without delay when the workup reveals a clear barrier that time will not fix. Both fallopian tubes blocked, a very low or absent sperm count, ovulation that does not occur without medical help, or a known genetic condition that a couple wishes to avoid passing on are all findings that shift the conversation from “keep trying” to “here is what treatment could offer.” Age plays a role too. Egg number and quality decline with age, a pattern the Mayo Clinic describes as the single strongest influence on IVF outcomes, so clinicians tend not to counsel long waits for people in their late thirties and beyond.

Others are asked to pause, and the reasons are usually protective rather than obstructive. An untreated thyroid disorder, uncontrolled diabetes or very high blood pressure can affect both the chance of conception and the safety of a pregnancy, so those are typically managed first. A newly diagnosed infection, such as chlamydia, is treated before any procedure involving the uterus. A polyp or fibroid found inside the uterine cavity may be removed before embryo transfer because it can interfere with implantation. Anyone with an active cancer diagnosis is generally directed to a fertility preservation pathway instead.

Weight sits in this category as well, and it deserves careful language. The NHS notes that both a very high and a very low body weight can disrupt ovulation and reduce the chance of conception, and some programs set a range within which they will proceed. This is about physiology and safety under sedation, not judgment. A clinician who raises it should also offer support to address it.

The decision to proceed, wait or explore alternatives always rests with the treating team, made together with the couple and revisited as results come in.

Fertility tests for the female partner: hormones and ovarian reserve

The blood tests for the female partner are trying to answer two questions. Is ovulation happening? And how many eggs are likely to respond if the ovaries are stimulated?

Ovulation is usually confirmed with a progesterone test drawn in the second half of the cycle. Progesterone is the hormone the ovary produces after releasing an egg; a rise means an egg was released. For people with irregular or absent periods, the NHS describes additional hormone checks, including follicle-stimulating hormone (FSH), luteinizing hormone (LH), thyroid function and prolactin. Thyroid and prolactin matter because either being out of range can suppress ovulation, and both are treatable causes that are easy to miss.

Ovarian reserve is a separate concept and often misunderstood. It describes the quantity of remaining eggs, not their quality and not the chance of conceiving this month. Two measures are common. Anti-Müllerian hormone (AMH) is a blood test that reflects the pool of small developing follicles; it can be drawn on any day. The antral follicle count (AFC) is an ultrasound count of those small follicles, ideally done early in the cycle. Together they help the team predict how the ovaries will respond to stimulation medicines and choose a plan that lowers the risk of ovarian hyperstimulation syndrome, an over-response that can cause fluid shifts and abdominal swelling.

A low AMH does not mean pregnancy is impossible, and a high one does not guarantee success. What it changes is strategy. Someone with a low reserve may be counseled that fewer eggs are likely per cycle; someone with a high reserve, common in polycystic ovary syndrome, may be given a gentler protocol. Any medicine choice, and every adjustment to it, is made by the prescribing clinician using these numbers.

Rubella immunity is also checked, since rubella infection in early pregnancy can harm the fetus and vaccination must happen before, not during, pregnancy.

What is a mock test in IVF? Tubes, uterus and the trial transfer

Anatomy tests look for physical barriers. The first is tubal patency, meaning whether the fallopian tubes are open. Two methods are common. A hysterosalpingogram (HSG) injects a dye visible on X-ray through the cervix and photographs it flowing through the tubes. A hysterosalpingo-contrast sonography (HyCoSy) does the same with an ultrasound-visible fluid. Both cause cramping similar to a period for a few minutes. If the tubes are blocked, IUI is off the table because sperm cannot reach the egg, and IVF, which bypasses the tubes entirely, becomes the logical route.

The uterine cavity is checked next. A transvaginal ultrasound, sometimes with saline infused to outline the cavity, can reveal fibroids, polyps or a septum, a wall of tissue dividing the space. A hysteroscopy, a thin camera passed through the cervix, gives a direct look and can remove small polyps at the same time. Laparoscopy, keyhole surgery under general anesthesia, is reserved for suspected endometriosis or pelvic scarring, per the NHS.

Then there is the mock embryo transfer, the “mock test” people ask about. It is a rehearsal, not a test of fertility. A clinician passes an empty catheter through the cervix into the uterus, noting the depth of the cavity and any curve or tightness in the cervical canal. On the real transfer day, that information helps the embryologist and physician place the embryo smoothly and quickly, with less manipulation of the cervix. Some programs perform it during the workup; others do it at egg retrieval while the patient is already sedated.

A related but different idea is the “mock cycle” for a frozen embryo transfer, where the lining is prepared with hormones and sometimes biopsied for endometrial receptivity testing. Here honesty matters: the evidence that receptivity testing improves outcomes for most patients is limited and inconsistent, and major guidelines do not recommend it routinely. If it is suggested, ask what specifically it is expected to change for you.

Tests for the male partner: what a semen analysis measures and what happens if it is abnormal

Male factors contribute to roughly half of couples’ difficulty conceiving, alone or alongside a female factor, yet the male workup is often the shortest part of the folder. Its centerpiece is the semen analysis.

The sample is produced by masturbation into a sterile container, usually at the clinic, after a short abstinence window. The World Health Organization’s laboratory manual recommends two to seven days without ejaculation before the sample; shorter and volume drops, longer and motility tends to fall. The lab then reports several values. Concentration is the number of sperm per unit volume. Motility is the proportion that are moving, and progressive motility the proportion moving forward purposefully. Morphology is the percentage with normal shape. Volume, pH and the presence of white blood cells, which may hint at infection, round out the report.

One result is never the whole story. Sperm production is sensitive to illness, fever, heat, stress and alcohol in the preceding weeks, so a single low reading may reflect a bad month rather than a fixed problem. The NHS advises repeating an abnormal analysis to confirm it before drawing conclusions.

If two samples are abnormal, the workup widens. Hormone tests including testosterone, FSH and LH help distinguish a problem in the testes from a signaling problem in the pituitary gland. A scrotal ultrasound can identify a varicocele, an enlarged vein that raises testicular temperature, or a blockage. Where sperm are entirely absent, genetic tests such as a karyotype, cystic fibrosis carrier screening and a Y-chromosome microdeletion test are considered, because certain genetic patterns can be passed to a son and change the counseling a couple receives.

What changes with the result? Mild reductions may still suit IUI, which concentrates the best sperm. Marked reductions often steer toward IVF with ICSI. Absent sperm may lead to surgical sperm retrieval or a conversation about donor sperm, decisions the team walks through step by step.

Tests both partners complete: infection screening, blood work and genetic carrier screening

The shared page of the folder is the one couples most often question. “Why does he need a hepatitis test for her procedure?” The answer is that gametes, embryos and laboratory staff all share a workspace, and a pregnancy is exposed to the health of both contributors.

Infection screening comes first. The NHS lists screening for HIV, hepatitis B and hepatitis C as standard before IVF, and most programs apply the same to IUI. A positive result does not necessarily end treatment; it changes how samples are handled and may prompt treatment of the infection first, with specialist input on reducing transmission risk to a partner or child. Syphilis is often added. Chlamydia, which can silently scar fallopian tubes, is tested with a urine sample or swab, and a positive result is treated in both partners before any uterine procedure.

General blood work follows: a full blood count to check for anemia, blood group and antibody screen in the female partner, and often a random glucose or HbA1c and thyroid function if not already done. Vitamin D is checked in some programs, although the evidence that correcting a low level improves fertility outcomes is not settled.

Genetic carrier screening is increasingly offered to both partners. It looks for recessive conditions, where a child is affected only if both parents carry a change in the same gene: cystic fibrosis, spinal muscular atrophy, thalassemia and sickle cell trait are common examples, with panels varying by ancestry and by program. A couple found to carry the same condition may be offered preimplantation genetic testing of embryos, prenatal testing, or donor gametes; a genetic counselor explains the options and the limits of each.

Cervical screening should be up to date, and any prescription medicines both partners take are reviewed by the clinician for pregnancy safety. Nothing here is decorative. Each result either clears a path or reveals something better dealt with now than midway through a cycle.

Artificial fertilisation preparation tests at a glance: who, what and when

A single table cannot capture every clinic’s protocol, and lists vary with history, age and the intended treatment. It can, however, show the shape of a typical workup and why the timing is so particular.

Test Who What it looks for Typical timing
Semen analysis Male partner Sperm count, motility, shape, volume Any day, after 2–7 days’ abstinence (WHO)
Progesterone Female partner Confirms ovulation occurred About 7 days before expected period
FSH, LH, AMH, thyroid, prolactin Female partner Ovulation signaling and ovarian reserve FSH/LH early in cycle; AMH any day
Transvaginal ultrasound with antral follicle count Female partner Ovarian reserve, cysts, fibroids, lining Early in cycle
HSG or HyCoSy Female partner Open or blocked fallopian tubes After bleeding stops, before ovulation
Mock embryo transfer Female partner (IVF) Cavity depth and cervical path During workup or at egg retrieval
HIV, hepatitis B and C, syphilis Both Blood-borne infection Any day; often repeated if results lapse
Chlamydia Both Silent infection that scars tubes Any day
Rubella immunity Female partner Protection before pregnancy Any day; vaccinate before treatment if needed
Genetic carrier screening Both Shared recessive conditions Any day; results take weeks

Two patterns stand out. Female hormone and anatomy tests are tied to the calendar of the cycle, which is why the workup rarely finishes in under a month. Shared and male tests are flexible, so completing them early avoids the common situation where every result is back except one, and a whole cycle is lost waiting.

Results also expire. Many programs require infection screening within a set period before treatment, so a couple who pauses for several months may be asked to repeat those bloods. It is worth asking at the outset how long each result remains valid.

How to prepare for IUI or IVF: the lifestyle steps the evidence supports

Once the tests are underway, most people want to do something. The honest list is shorter than the internet suggests, but the items on it are backed by mainstream guidance.

Folic acid is the clearest. The NHS and the NIH Office of Dietary Supplements recommend that anyone who could become pregnant take a daily folic acid supplement before conception and through early pregnancy, because it lowers the risk of neural tube defects such as spina bifida. Your clinician will specify the product and amount appropriate for you; some people with a history of neural tube defects or certain medicines are advised differently.

Smoking affects both partners. The NHS notes it reduces fertility in women and harms sperm quality in men, and stopping before treatment is one of the few changes with a measurable effect. Alcohol is next: the NHS advises against drinking while trying to conceive, and heavy drinking lowers sperm quality. On caffeine, the evidence is weaker; the NHS states there is no clear link between moderate caffeine and reduced fertility, so a morning coffee is not the villain it is sometimes made out to be.

For the male partner, avoiding prolonged heat to the testes, such as frequent hot tubs, is reasonable, since sperm develop best a little below body temperature. Recreational drugs, anabolic steroids and some prescription medicines affect sperm production; disclose everything to the clinician rather than stopping anything on your own.

Vaccinations are best completed before a cycle. Rubella and varicella vaccines are live and cannot be given in pregnancy, so any gaps identified in screening are filled during the workup.

Movement and sleep help in the ordinary ways. Supplements marketed as fertility boosters generally lack good evidence, and some interfere with the medicines used in treatment. Bring the bottles to your appointment so the team can review them.

What the following days and weeks usually look like once testing is complete

With results in hand, the plan has a shape, and the timeline depends on which route was chosen.

For IUI, the cycle begins with the period. If medicines are used to encourage a follicle to mature, monitoring ultrasounds track its growth. Insemination is timed to ovulation, either detected with urine LH tests or triggered with an injection; the Mayo Clinic describes the procedure itself as taking a few minutes, after which the person lies down briefly and then resumes normal activity. Mild cramping or light spotting for a day is common. A pregnancy test is done about two weeks later, according to the Cleveland Clinic; testing earlier can give misleading results, particularly after a trigger injection.

IVF runs longer and more intensely. The Mayo Clinic puts one full cycle at roughly two to three weeks from the start of stimulation to embryo transfer. Stimulation involves daily injections and several monitoring visits for ultrasound and blood tests. Egg retrieval is scheduled about 34 to 36 hours after a final trigger injection and performed under sedation, with cramping and bloating for a few days afterward. Fertilization is confirmed the next day, and transfer of an embryo typically happens two to five days later, or embryos are frozen for transfer in a later cycle. A blood pregnancy test follows roughly two weeks after retrieval.

Emotionally, these weeks are often harder than the tests. The waiting period between transfer and test is short on the calendar and long in the mind. Many programs offer counseling; using it is not a sign of struggling but a sensible part of the plan.

Every figure above is a typical range, not a promise. Cycles are adjusted, delayed or occasionally canceled based on how the body responds, and those decisions belong to the treating team.

What people often get wrong about tests before IUI and IVF

Fertility gathers myths the way a kitchen gathers magnets. A few deserve direct correction.

“If the woman’s tests are normal, the problem must be hers anyway.” The opposite is closer to the truth. Male factors contribute in around half of couples with difficulty conceiving, which is why the semen analysis is requested at the first visit rather than after the female workup is exhausted.

“AMH tells me whether I can get pregnant.” AMH estimates how many eggs remain, not how good they are or whether one will meet a sperm this month. People with low AMH conceive naturally; people with high AMH do not always respond as hoped. It is a planning number, not a verdict.

“One semen analysis is enough.” Sperm production fluctuates with illness, heat and stress over the preceding weeks, and the NHS advises confirming an abnormal result with a repeat sample before acting on it.

“The mock transfer tests whether an embryo will implant.” It measures the route, not the destination. Its purpose is a smoother real transfer, nothing more.

“An HSG is a treatment, so I can skip trying afterward.” Some studies suggest a modest rise in natural conception in the months after tubal flushing, but it is a diagnostic test first, and the evidence for a treatment effect is limited and mixed.

“Infection screening means they suspect something about me.” It is universal, applied to every person and every sample, for laboratory safety and for the future child.

“Bed rest after insemination or transfer improves the odds.” Guidance from the Mayo Clinic is to resume normal activity; prolonged rest has not been shown to help and can add anxiety.

Believing the myths is not a character flaw. They persist because they offer something concrete to hold onto. The tests offer something better: information about your own body, specific enough to plan around.

Questions to ask your care team about your artificial fertilisation preparation tests

A good results appointment is a two-way conversation, and it helps to arrive with questions written down. These are the ones that tend to unlock the most useful answers.

  • Which of my results changed the plan, and which were simply reassuring? This separates the findings that matter from the ones that are noise.
  • Is there anything you would want to treat, remove or repeat before we start a cycle, and why?
  • Which treatment route are you recommending for us, and what in our results points to it rather than the alternatives?
  • How does my ovarian reserve shape the protocol you have in mind, and what are you doing to lower the risk of over-response?
  • Should the semen analysis be repeated, and is a referral to a specialist in male fertility appropriate?
  • Do any of our infection or genetic results need specialist counseling before we proceed?
  • How long are these results valid, and which ones will need repeating if we pause?
  • Will a mock transfer be done, and when? If a receptivity test or another add-on is suggested, what is the evidence it would change our outcome specifically?
  • What is the realistic timeline for us from today to a first cycle, given the tests still pending?
  • What lifestyle changes, if any, would you actually expect to make a difference in our case?
  • Who do we call, and at what hours, if something feels wrong during the cycle?
  • What support is available for the emotional side of this, and how do we access it?

Take notes, or ask whether a partner or friend can join. Ask the clinician to write down any numbers you are given, along with what a normal range looks like. If an answer is uncertain, it is fair to ask what the evidence shows and what remains unknown; a careful team will tell you.

The point of these questions is not to challenge the plan but to own it. Treatment goes better when both partners understand why each step is there.

When to call your doctor

Most of the preparation tests are low-risk, and mild cramping, spotting or bruising at a blood-draw site are expected. A few situations, however, should prompt a call to the clinic or, out of hours, urgent care. Contact your team the same day if you notice any of the following after an HSG, HyCoSy, hysteroscopy or mock transfer.

  • Fever, chills or a foul-smelling vaginal discharge, which can signal infection introduced during a uterine procedure.
  • Heavy vaginal bleeding that soaks a pad within an hour, or bleeding that continues beyond a couple of days.
  • Pelvic pain that is severe, worsening, or not eased by simple measures, particularly if accompanied by nausea or feeling faint.
  • Any allergic-type reaction after contrast dye: hives, swelling of the face or lips, wheezing or difficulty breathing. Breathing difficulty is an emergency; call emergency services.

Once a cycle begins, the red flags shift. The Mayo Clinic describes ovarian hyperstimulation syndrome as a recognized risk of stimulation medicines. Seek urgent advice for rapid abdominal swelling, marked weight gain over a day or two, severe bloating with vomiting, breathlessness, or passing very little urine. After egg retrieval, severe one-sided pain, dizziness or fainting can indicate bleeding or ovarian torsion, a twisting that needs prompt assessment.

For the male partner, pain, swelling or redness of the testicle, or fever after a scrotal ultrasound or biopsy, should be reported the same day.

Emotional red flags matter too. Persistent low mood, hopelessness, or thoughts of self-harm during treatment are reasons to contact your team or a mental health service without waiting for the next appointment.

When in doubt, call. Fertility teams expect these calls, and none of the situations above becomes easier to manage by waiting until morning. Every decision about pausing, adjusting or continuing treatment rests with the clinicians who know your results.

Frequently asked questions

What are the tests done before IUI?

Before IUI, the male partner has a semen analysis, and the female partner has hormone blood tests to confirm ovulation and estimate ovarian reserve, a pelvic ultrasound, and usually a tubal patency check such as an HSG or HyCoSy, because IUI needs at least one open tube. Both partners complete infection screening for HIV, hepatitis B and C, and chlamydia, and rubella immunity is checked in the female partner.

What are the four types of artificial insemination?

The four are intrauterine insemination (IUI), where washed sperm is placed in the uterus; intracervical insemination, where sperm is placed at the cervix; intravaginal insemination, used in some at-home donor arrangements; and intratubal insemination, where sperm is placed in a fallopian tube, now rarely used. In clinical practice, artificial insemination almost always means IUI, which the NHS and Mayo Clinic describe as a brief procedure timed to ovulation.

How to prepare for artificial insemination or IUI?

Complete every requested test early, especially the flexible ones like semen analysis and infection bloods, so a cycle is not lost waiting on one result. Take a daily folic acid supplement as advised by your clinician, stop smoking, avoid alcohol, and bring any supplements or medicines to your appointment for review. Confirm rubella vaccination status beforehand, and ask the team exactly how ovulation will be timed and when to test for pregnancy.

What is a mock test in IVF?

A mock, or trial, embryo transfer is a rehearsal in which a clinician passes an empty catheter through the cervix into the uterus to measure cavity depth and note any curve or tightness in the canal. It helps the real transfer go smoothly with minimal manipulation. It does not assess fertility or predict implantation. Some programs do it during the workup; others perform it at egg retrieval under sedation.

What is a mock embryo transfer compared with a mock cycle?

A mock embryo transfer is a one-time anatomical rehearsal with an empty catheter. A mock cycle is different: the uterine lining is prepared with hormones as it would be for a frozen embryo transfer, sometimes with a biopsy for endometrial receptivity testing. Evidence that receptivity testing improves outcomes for most patients is limited and inconsistent, so it is worth asking what a mock cycle is expected to change in your specific situation.

Which fertility tests for couples are done on both partners?

Both partners are usually screened for HIV, hepatitis B, hepatitis C and often syphilis and chlamydia, since gametes and embryos share a laboratory space and a pregnancy is exposed to the health of both contributors. Genetic carrier screening for recessive conditions such as cystic fibrosis may be offered to both. General blood work, a medicine review, and counseling are also commonly shared steps.

Why does the male partner need tests before IVF if the problem seems to be with the female partner?

Because male factors contribute in about half of couples with difficulty conceiving, and they often exist alongside a female factor rather than instead of one. A semen analysis can change the treatment route, for example from standard IVF to ICSI. Infection screening applies to every person whose sample enters the laboratory. Skipping the male workup risks choosing a treatment that does not fit the actual problem.

How long do the preparation tests take before treatment can start?

Typically one to two menstrual cycles, because several female hormone and anatomy tests must be done on specific days: FSH and LH early in the cycle, a tubal patency check after bleeding stops but before ovulation, and progesterone about a week before the next period. Semen analysis and infection bloods can be done any day. Genetic carrier screening results often take several weeks to return. Clinics vary, so ask for your expected timeline.

What does a low AMH result mean for IVF?

A low anti-Müllerian hormone level suggests a smaller pool of remaining eggs and usually predicts fewer eggs collected per stimulation cycle. It does not measure egg quality and does not mean pregnancy is impossible; people with low AMH conceive both naturally and with treatment. Clinicians use it mainly to plan the stimulation protocol and set realistic expectations, and the decision on approach rests with the treating team.

Do test results expire before fertility treatment?

Many do. Infection screening for HIV and hepatitis is commonly required within a set window before a cycle, so a pause of several months may mean repeating those bloods. Semen analyses and hormone tests may also be repeated if much time has passed or circumstances have changed. Ask your team at the outset how long each result remains valid so you can plan repeat tests without losing a cycle.

References

This article is for general information only and is not a substitute for professional medical advice. Please consult a qualified doctor about your individual situation.

Dr. Şule Eren
Dr. Şule Eren, MD
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Published October 26, 2026 Last updated September 20, 2026
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